abstract：:Multiplication of virulent and avirulent Leishmania donovani promastigotes was inhibited by brain heart infusion broth (BHI) (3.7%) supplemented with haemolysed or whole blood (2.5-10%) or heat-inactivated foetal bovine serum (HIFBS) (10-20%). Peptone, glutamine, tryptophan, purine bases, ribonucleosides, deoxyribonuc...

journal_title：Parasitology

authors： Kar K

更新日期：1997-09-01 00:00:00

abstract：:We estimate the incidence rate, serological conversion rate and basic case reproduction number (R0) of Leishmania infantum from a cohort study of 126 domestic dogs exposed to natural infection rates over 2 years on Marajó Island, Pará State, Brazil. The analysis includes new methods for (1) determining the number of s...

journal_title：Parasitology

authors： Quinnell RJ,Courtenay O,Garcez L,Dye C

更新日期：1997-08-01 00:00:00

abstract：:Two cDNAs encoding cysteine proteinases were isolated from a cDNA library constructed from feeding females of Heterodera glycines. The library was screened with a cysteine proteinase gene fragment originally amplified from cDNA of H. glycines. Database searches predict that 1 cDNA (hgcp-I) encodes a cathepsin L-like p...

journal_title：Parasitology

authors： Urwin PE,Lilley CJ,McPherson MJ,Atkinson HJ

更新日期：1997-06-01 00:00:00

abstract：:A 30-h in vitro susceptibility test of Plasmodium falciparum wild isolates to artemisinin, chloroquine, sulfadoxine/pyrimethamine and mefloquine was performed in Kibaha, Tanzania. A sigmoid Emax model was fitted to all data for each isolate and drug combination. Artemisinin and mefloquine exhibited 100% growth inhibit...

journal_title：Parasitology

authors： Alin MH

更新日期：1997-06-01 00:00:00

abstract：:An 8-point visual index was developed for Oil Red O staining of neutral lipids in infective juveniles (IJs) of Steinernema carpocapsae (A11), S. riobravis (Biosys 355), S. feltiae (UK76) and S. glaseri (NC). The visual index was found to be a reliable and rapid method for determining the relative neutral lipid content...

journal_title：Parasitology

authors： Patel MN,Stolinski M,Wright DJ

更新日期：1997-05-01 00:00:00

abstract：:In areas where malaria is endemic, helminthic infections, caused by intestinal or filarial parasites, commonly coexist with malaria in the same individual. This study investigates the course of Plasmodium berghei malaria infection in CBA/J mice inoculated with irradiated attenuated 3rd-stage larvae (L3) of Brugia paha...

journal_title：Parasitology

authors： Yan Y,Inuo G,Akao N,Tsukidate S,Fujita K

更新日期：1997-04-01 00:00:00

abstract：:The 4-aminoquinolines chloroquine (CQ) and amodiaquine (AM) were used to treat Gambian children with uncomplicated falciparum malaria in a randomized drug trial. Blood samples were taken immediately before treatment (day 0), and at day 7 and day 28 after treatment. Samples from those parasitologically positive at day ...

journal_title：Parasitology

authors： Duraisingh MT,Drakeley CJ,Muller O,Bailey R,Snounou G,Targett GA,Greenwood BM,Warhurst DC

更新日期：1997-03-01 00:00:00

abstract：:Schistosoma mansoni egg counts by faecal examination vary considerably and are not very sensitive, so prevalences are underestimated. The distribution of egg counts can adequately be described by a stochastic model which distinguishes variation in counts between persons and variation in repeated counts within a person...

journal_title：Parasitology

authors： De Vlas SJ,Engels D,Rabello AL,Oostburg BF,Van Lieshout L,Polderman AM,Van Oortmarssen GJ,Habbema JD,Gryseels B

更新日期：1997-02-01 00:00:00

abstract：:We examine the evolution of microparasites in response to the immune system of vertebrate hosts. We first describe a simple model for an acute infection. This model suggests that the within-host dynamics of the microparasite will be a 'race' between parasite multiplication and a clonally expanding response by the host...

journal_title：Parasitology

authors： Antia R,Lipsitch M

更新日期：1997-01-01 00:00:00

abstract：:A metabolic pathway known as the mannitol cycle has been identified in Eimerian parasites. The pathway is a shunt off of the glycolytic pathway at fructose-6-phosphate (F6P). Two enzymes convert F6P to mannitol and two other enzymes are responsible for converting mannitol back to F6P when it is utilized. Although the ...

journal_title：Parasitology

authors： Schmatz DM

更新日期：1997-01-01 00:00:00

abstract：:Intra-erythrocytic stages of malaria parasites can alter the surface of their host cells and release toxins which induce the production of cytokines, which in turn can up- or down-regulate the expression of adhesion receptors on the surface of microvascular endothelial cells. New adhesion receptors on endothelial cell...

journal_title：Parasitology

authors： Hommel M

更新日期：1997-01-01 00:00:00

abstract：:Oocysts of Cryptosporidium parvum showed relatively low levels of SOD activity. The SOD which had a pI of 4.8 and an approximate molecular weight of 35 kDa appeared to be iron dependent. Catalase, glutathione transferase, glutathione reductase and glutathione peroxidase activity could not be detected, nor could trypan...

journal_title：Parasitology

authors： Entrala E,Mascaro C,Barrett J

更新日期：1997-01-01 00:00:00

abstract：:Trichinella spiralis larvae infect and develop within skeletal muscle cells causing major changes to their mechanical properties. The aim of this investigation was to determine the effects of T. spiralis on the power output and fatigue resistance of the mammalian diaphragm under conditions simulating in vivo operation...

journal_title：Parasitology

authors： Harwood CL,Young IS,Lee DL,Altringham JD

更新日期：1996-12-01 00:00:00

abstract：:Theileria annulata infects and reversibly transforms bovine leucocytes. The parasite-transformed cells are immortalized, metastatic and express a number of metalloproteinases including matrix metalloproteinase 9 which they secrete. All the metalloproteinases observed on substrate gels are inhibited by tissue inhibitor...

journal_title：Parasitology

authors： Adamson RE,Hall FR

更新日期：1996-11-01 00:00:00

abstract：:A large database on the transmission of a stabilate of the Theileria parva Muguga stock from one breed of cattle using two stocks of Rhipicephalus appendiculatus, Muguga and Ol Pejeta was developed and analysed. Factors associated with the ticks and cattle, and the infections developing in cattle were studied in relat...

journal_title：Parasitology

authors： Young AS,Dolan TT,Morzaria SP,Mwakima FN,Norval RA,Scott J,Sherriff A,Gettinby G

更新日期：1996-09-01 00:00:00

abstract：:Little is known about the effects of infection with Borrelia burgdorferi, the bacterium that causes Lyme disease, on its tick vectors. The purpose of this study was to determine the behavioural and ecological effects of infection by the bacterium in nymphal and adult black-legged (Ixodes scapularis) ticks. We found th...

journal_title：Parasitology

authors： Lefcort H,Durden LA

更新日期：1996-08-01 00:00:00

abstract：:A field trial was conducted to evaluate the potential of the nematode-destroying fungus Duddingtonia flagrans to control free-living stages of horse strongyles. In late Spring 2 groups of horses (yearlings) with mixed infections of strongyles were allowed to contaminate 2 equal-sized pastures. One of the groups (F) re...

journal_title：Parasitology

authors： Larsen M,Nansen P,Grøndahl C,Thamsborg SM,Grønvold J,Wolstrup J,Henriksen SA,Monrad J

更新日期：1996-07-01 00:00:00

abstract：:Infection of intermediate hosts with eggs of taeniid parasites results in a larval infestation known as cysticercosis. A number of studies have indicated that cysticercosis is associated with immunosuppression, although little is known about the mechanisms involved. In the present study, mice infected with the larvae ...

journal_title：Parasitology

authors： Villa OF,Kuhn RE

更新日期：1996-06-01 00:00:00

abstract：:Expression of metalloprotease activities during metacyclogenesis of a series of strains and clones of Trypanosoma cruzi was investigated using SDS-polyacrylamide gel electrophoresis with a range of different co-polymerized proteolytic substrates. A complex pattern of metalloprotease expression, with considerable quant...

journal_title：Parasitology

authors： Lowndes CM,Bonaldo MC,Thomaz N,Goldenberg S

更新日期：1996-04-01 00:00:00

abstract：:Vitellogenin synthesis by the fat body has been monitored using in vitro culture and immunoprecipitation. This system was found to be efficient for measuring vitellogenin production in both non-infected Tenebrio molitor and those infected with Hymenolepis diminuta. In fat bodies from infected beetles, vitellogenin pro...

journal_title：Parasitology

authors： Webb TJ,Hurd H

更新日期：1996-04-01 00:00:00

abstract：:In order to study the effects of acclimatization of Plasmodium in beta-thalassaemic mice, we used a mouse model of beta-thalassaemia (DBA/2J/beta-thal/beta-thal), similar to that observed in humans. We acclimatized 3 rodent malarias (P. berghei, P. chabaudi and P. yoelii) in DBA/2J and DBA/2J/beta-thal mice lines, by ...

journal_title：Parasitology

authors： Clarebout G,Gamain B,Slomianny C,Camus D,Dive D

更新日期：1996-03-01 00:00:00

abstract：:A rapid, visually read, dot-ELISA developed for the detection and differentiation of trypanosome species in tsetse flies (Glossina spp.), was field tested alongside the standard fly dissection method on a range in south eastern Kenya. Of 104 G. pallidipes dissected, 2 were found to be infected with trypanosomes in the...

journal_title：Parasitology

authors： Bosompem KM,Masake RA,Assoku RK,Opiyo EA,Nantulya VM

更新日期：1996-02-01 00:00:00

abstract：:The radiation-attenuated schistosome vaccine induces a high level of protective immunity in rodents. In order to assess its potential relevance to man, we have tested its efficacy in the non-human primate Papio anubis. A vaccination regime consisting of 3 exposures of approximately 9000 cercariae irradiated with 30 or...

journal_title：Parasitology

authors： Yole DS,Pemberton R,Reid GD,Wilson RA

更新日期：1996-01-01 00:00:00

abstract：:Analysis of the electrical properties of neurons in the motornervous system of Ascaris sutom suggests that it is largely an analogue system. The motorneurons do not conduct action potentials and they release transmitter tonically at their normal resting potential; transmitter release is increased or decreased as a con...

journal_title：Parasitology

authors： Davis RE,Stretton AO

更新日期：1996-01-01 00:00:00

abstract：:Sequence comparisons have recently shown that the Schistosoma mansoni protein Sm32 is similar to asparaginyl endoproteinases, a novel family of cysteine proteinases, of which the legumains from legumes are the best characterized. By synthesizing and employing fluorogenic peptide substrates for the specific detection o...

journal_title：Parasitology

authors： Dalton JP,Hola-Jamriska L,Brindley PJ

更新日期：1995-12-01 00:00:00

abstract：:An apparatus for artificial feeding of Rhipicephalus appendiculatus nymphae was modified to improve feeding performance. Heparinized blood was supplied above a treated artificial membrane while the ticks attached below on its undersurface. The feeding apparatus was incubated at 37 degrees C in an atmosphere of 3% CO2 ...

journal_title：Parasitology

authors： Waladde SM,Young AS,Mwaura SN,Njihia GN,Mwakima FN

更新日期：1995-11-01 00:00:00

abstract：:Glycans at the surface of adult Schistosoma mansoni were investigated with gold-labelled lectins. The fragile complex of the glycans with the outer membranes could be preserved for electron microscopy by avoiding extensive pre-fixation with aldehydes and by introducing osmium-ferrocyanide as a membrane fixative. Male ...

journal_title：Parasitology

authors： Schmidt J

更新日期：1995-09-01 00:00:00

abstract：:A guinea-pig immunoglobulin-G linked agarose column was used to detect the immunoglobulin-G binding proteins (IGBP) in the ixodid ticks, Rhipicephalus appendiculatus, Amblyomma variegatum and Ixodes hexagonus. IGBPs were found in salivary gland extract (SGE) of the unfed ticks of all 3 species. The sizes of the IGBPs,...

journal_title：Parasitology

authors： Wang H,Nuttall PA

更新日期：1995-08-01 00:00:00

abstract：:SIV rats infected with a high dose (50,000 oocysts) of Eimeria nieschulzi displayed clinical symptoms of coccidiosis such as diarrhoea (days 6 and 7 post-primary infection) and weight loss (days 6-8 post-primary infection) and were completely immune to challenge with a similar dose. The ability of rats to produce tumo...

journal_title：Parasitology

authors： Smith NC,Ovington KS,Deplazes P,Eckert J

更新日期：1995-07-01 00:00:00

abstract：:Aspects of vitellogenesis, known to be controlled by juvenile hormone, are adversely affected by Hymenolepis diminuta infection of Tenebrio molitor, in spite of circulating titres of the hormone remaining unchanged. It has therefore been proposed that juvenile hormone binding is disrupted at the tissue site level. Juv...

journal_title：Parasitology

authors： Webb TJ,Hurd H

更新日期：1995-06-01 00:00:00

abstract：:In a population of copepods (Cyclops strenuus abyssorum), with a naturally high prevalence of infection with procercoids of Diphyllobothrium spp., no difference in body size was found between infected and uninfected hosts. However, a significant reduction in the reproductive capacity of infected females was observed, ...

journal_title：Parasitology

authors： Pasternak AF,Huntingford FA,Crompton DW

更新日期：1995-05-01 00:00:00

abstract：:The behavioural responses of Onchocerca lienalis microfilariae (mf) to tissue factors of the surrogate black fly host, Simulium vittatum, were studied using a novel in vitro bioassay. Mf accumulated towards thoracic tissues to a density 4 times higher than towards abdominal tissues, despite the larger surface area and...

journal_title：Parasitology

authors： Lehmann T,Cupp SM,Cupp WE

更新日期：1995-04-01 00:00:00

abstract：:Patterns of genetic subdivision in parasite populations can provide important insights into transmission processes and complement information obtained using traditional epidemiological techniques. We describe mitochondrial sequence variation in 265 Ascaris collected from 62 individual hosts (humans and pigs) from 35 h...

journal_title：Parasitology

authors： Anderson TJ,Romero-Abal ME,Jaenike J

更新日期：1995-02-01 00:00:00

abstract：:Small vessels showing inflammatory cell infiltrates were invariably observed in the vicinity of intact Toxoplasma tissue cysts within the brains of mice congenitally infected with the protozoan. Lymphocytes were observed in intimate contact with the luminal aspect of the endothelium, penetrating into the thickened bas...

journal_title：Parasitology

authors： Sims TA,Hay J

更新日期：1995-02-01 00:00:00

abstract：:The amitochondriate parasitic protist Trichomonas vaginalis can utilize either glucose or maltose as carbon and energy source. The mechanisms of maltose utilization were explored with uptake experiments using radio-isotope labelled maltose in combination with the silicone-oil centrifugation technique and enzymatic ass...

journal_title：Parasitology

authors： ter Kuile BH,Müller M

更新日期：1995-01-01 00:00:00

abstract：:Infectious agents have considerable potential to regulate or constrain the population growth of vertebrate hosts in natural habitats. A broad theoretical framework provides many insights into how the biology of the parasite and the demography of the host interact to determine this impact. It may manifest itself as a s...

journal_title：Parasitology

authors： Anderson RM

更新日期：1995-01-01 00:00:00

abstract：:The development of a successful vaccine depends not only on the production of the vaccine itself, but also on the design pf the vaccination programme. This involves a better understanding of the epidemiology of the disease to be controlled, the delivery system to be used and the costs involved. This review focuses on ...

journal_title：Parasitology

authors： Bundy DA,Chan MS,Guyatt HL

更新日期：1995-01-01 00:00:00

abstract：:Trials were carried out to study the humoral immune response of blackflies to filariae following infection using the intrathoracic injection technique. An induced 66 kDa protein was abundant in the haemolymph of the European species Simulium ornatum following infection with bovine Onchocerca lienalis. This protein was...

journal_title：Parasitology

authors： Hagen HE,Grunewald J,Ham PJ

更新日期：1994-12-01 00:00:00

abstract：:Infection of breeding hens with Eimeria maxima induces production of Eimeria-specific IgG antibodies which are transferred to hatchlings via the egg yolk and confer a high degree of maternal immunity against homologous challenge and partial immunity to infection with another important species, Eimeria tenella. As an e...

journal_title：Parasitology

authors： Smith NC,Wallach M,Petracca M,Braun R,Eckert J

更新日期：1994-12-01 00:00:00

abstract：:A multi-subunit antigen (native M(r) > 200 kDa, reduced M(r) 97-100 kDa) has been identified in homogenates of Trichinella spiralis larvae using affinity-purified rabbit anti-keyhole limpet haemocyanin (KLH) antibodies and its cross-reactivity with KLH was confirmed by competition blotting. The antigen was not present...

journal_title：Parasitology

authors： Modha J,Robertson WM,Kennedy MW,Kusel JR

更新日期：1994-11-01 00:00:00